Recently, metabolomics has demonstrated it can capture the cumulative effect of the environment on health and disease. It can therefore ideally complement genomics to assess someone’s health status and predict the onset of diseases. So far, there is not 1 generic method to capture the diverse and complex metabolome due to the large variation of physicochemical properties and concentration of metabolites present in biological samples. Therefore, a combination of metabolic profiling methods is often used and a proper study design is important to answer a biological question.
To use metabolomics to detect the onset of diseases, reveal disease mechanisms and choose the proper prevention and treatment strategy, X-omics will address the following main challenges:
Higher throughput, lowering costs and amount of sample needed to enable population-based metabolomics analyses.
Absolute quantitation for more metabolites enabling the combination of different studies and additional data analysis strategies such as fluxomics.
Miniaturizing metabolomics technology to enable analysis of organoids, organ-on-a-chip and single cell samples.
Developing technologies to study the dynamics within and between cells and tissues and the human body.
The main area of expertise of the metabolomics facilities, that are part of the X-omics research infrastructure, consists of state-of-the-art metabolomics, lipidomics and inborn errors of metabolism. The services offered by the facilities are described in more detail below.
- Experimental design
- Technical assistance
Sample preparation and storage:
- Automated blood sampling and storage
- Global deep metabolic profiling (RP or HILIC)
- Untargeted exploratory lipodomics
- Multiple metabolite panels (bile acids, oxylipids, amino acids, acylcarnitines, fatty acids, sterols, etc.)
- Quantative lipodomics for >1000 lipid species in 22 lipid classes
Metabolic flux analysis
- Statistical analysis
- Metabolite identification
Available equipment for these analyses:
- UPLC-High resolution MS (Qtrap 6500, TT6600, QTOF)
- Lipidyzer platform
All services mentioned above are custom services.
Please contact our helpdesk for a quote with turnaround times and prices based on your research question.